ABSTRACT
Objective To observe the clinical effect of treating diabetic nephropathy with Shengqidihuang decoction combined with metformin. Methods 54 cases with diabetic nephropathy were randomly divided into two groups by means of random number table. Both groups were given diabetic routine treatment. The control group was treated with metformin, 0.25 mg each time, three times a day, based on the diabetic routine treatment for 8 weeks, and the treatment group was treated with Shengqidihuang decoction, once a day, 4 weeks as a course, continuously treated for 2 courses based on the control group. 24 h urinary protein (24hUAE) , serum creatinine (SCr) , blood urea nitrogen (BUN) and fasting blood glucose (FBG) were detected before and after the treatment. The clinical effect was observed between the two groups after the treatment. Results The total effective rate of the treatment group and the control group was 85.2% and 66.7% respectively, showing a significant difference (λ2=3.376, P<0.05). Compared with the control group, 24 hUA ,SCr, BUN and FBG in the treatment group decreased significantly (λ2=4.231, P<0.05) after the treatment.24hUAE, SCr, BUN and FBG decreased significantly after the treatment, especially in the treatment group, also showing a significant difference (λ2= 3.754, P<0.05). Conclusion The therapy of mefformin combined with Shengqidihuang decoction on diabetic nephropathy was better than metformin only.
ABSTRACT
AIM: To investigate the changes of oxidative stress in the kidneys and their roles in nephropathy in diabetic rats. METHODS: Diabetic rats were induced by streptozotocin (STZ). 36 rats were divided into three groups randomly: (1) NC group, normal control rats; (2) DM group, diabetic rats received protamine zinc insulin (PZI) 2U-4U/2 d; (3) DT group, diabetic rats received PZI 9-12 U/kg body weigh/day. 12 weeks later, rats were killed, blood glucose, blood cholesterol, serum creatinine, blood urea nitrogen, HbA1c, urinary creatinine, and urinary protein for 24 h were measured. The activities of antioxidant enzymes in renal cortex, including total superoxide dismutase (TSOD), Cu-Zn superoxide dismutase (Cu-Zn SOD), Mn superoxide dismutase (Mn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and maleic dialdehyde (MDA) were measured by chromatometry. RT-PCR was performed to detect the expression of different antioxidant enzymes mRNA. RESULTS: For all the targets we measured, there was no significant difference between NC and DT groups. Compared with the other two groups, the levels of blood glucose, cholesterol, trigalloyl glycerol, HbA1c in DM group increased significantly. The activities of TSOD, Cu-Zn SOD and CAT decreased significantly. The activity of GSH-Px increased significantly. There was no significant difference among the activities of Mn SOD in all three groups. The level of MDA in DM group was much higher than that in NC or DT group. The relative expression levels of GSH-Px and Cu-Zn SOD mRNA in DM group were higher than those in other two groups, while the relative expression level of CAT decreased. Mn SOD mRNA was expressed without significant difference in all groups. Compared with NC or DT group, urinary protein in DM group increased significantly, while creatinine clearance rate decreased. CONCLUSIONS: Hyperglycemia affected the expression of antioxidant enzymes. Oxidative stress was caused by hyperglycemia in diabetic rats and may be an important factor in the etiology of diabetic nephropathy.